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From: "Gillian Millburn (Genetics)" <gm119@gen.cam.ac.uk>
Reply-To: "Gillian Millburn (Genetics)" <gm119@gen.cam.ac.uk>
Subject: Re: pUAST derivative
To: bionet-drosophila@net.bio.net, glaser@wadsworth.org
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Date: Tue, 25 Jan 2005 16:54:23 +0000 (GMT)
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Hi Robert,

there is a vector that might be useful to you - it is "UAS-Stinger"
which was described in:

Barolo et al., 2000
GFP and beta-galactosidase transformation vectors for promoter/enhancer
analysis in Drosophila. BioTechniques 29(4): 726--732

and is also in GenBank as AF242366.

The vector has a UAS-nuclearEGFP marker which is flanked by Su(Hw) binding
sites and also a white+ marker. I don't know whether you could chop out the
GFP and put in whatever coding region you wanted ?

The FlyBase pages with all the info we have about this construct are:

http://fbserver.gen.cam.ac.uk:7081/.bin/fbidq.html?FBtp0013491

and

http://fbserver.gen.cam.ac.uk:7081/.bin/fbidq.html?FBmc0002845

(this second page lists the restriction sites present in the construct
which are described in the BioTechniques paper)

hope that is helpful,

Gillian


--------------------------------------------------------------
Gillian Millburn.

FlyBase (Cambridge),
Department of Genetics,
University of Cambridge,
Downing Street,                       email: gm119@gen.cam.ac.uk
Cambridge,  CB2 3EH,                  Ph : 01223-333963
UK.                                   FAX: 01223-333992

FlyBase: http://fbserver.gen.cam.ac.uk/
--------------------------------------------------------------


> 
> Is anyone aware of a pUAST derivative containing Su(Hw) binding sites 
> flanking the 5xUAS-hsp70pro sequences to minimize background 
> expression from UAS insertions?
> -- 
> 
> 
> Robert L. Glaser, Ph.D.
> Wadsworth Center, Div. Genetic Disorders
> New York State Department of Health &
> University at Albany, State University of New York
> P. O. Box 22002
> Albany, NY  12201-2002
> 
> phone:	518-473-4201
> fax:	518-474-3181
> e-mail:	glaser@wadsworth.org
> www.wadsworth.org/resnres/bios/glaser.htm
> ---
> 


.