From owner-celegans@net.bio.net Mon Oct 13 23:00:00 1997
Path: biosci!biosci!not-for-mail
From: daemon@net.bio.net
Newsgroups: bionet.celegans
Subject: longevity in C. elegans 8
Date: 14 Oct 1997 06:26:24 -0700
Organization: Private Person
Lines: 61
Approved: wmorgan@acs.wooster.edu
Distribution: world
Message-ID: <61vru0$qlt@net.bio.net>
NNTP-Posting-Host: net.bio.net
Summary: no
Keywords: longevity, C. elegans

>From The Worm Breeder's Gazette, 1996, v. 14, No. 4, p. 50.

AN ATTEMPT TO SLOW AGING IN  C.  elegans.  5.THE  EFFECT  OF
       ROYAL JELLY EXTRACT WATER SOLUIONS IN DIFFERENT
                     CONCENTRATIONS
   Vladimir V.  Bakaev, box 45, Novosibirsk, 630107, USSR

The purpose  of this study was the effect of different concent-
rations of royal jelly extract on nematode life span.  In  this
experiment royal jelly extract was used in the form of "Apilac-
tosa" (produced by "Fertane",  Moscow, Nagatinskaya str., 1) in
following  dilutions  :  1:10(3),  1:10(4),  1:10(5),  1:10(6),
1:10(7),  1:10(8), 1:10(9) and 1:10(10). Three adult animals (3
-  5 days old) were kept in microtitre wells containing 0,75 ml
of liquid medium (with E. coli and without royal jelly extract)
during 4 hours, then they were discarded and newborn larvae we-
re transferred in next wells (with royal jelly extract  in  any
concentration) every day (one worm in one well).  This investi-
gation was carried out in temperature +21oC and  in  the  dark-
ness.  The obtained results are presented in the following tab-
le.


=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E0=C4=
=C4=C4=C4=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
Concentration  of royal=C5     =C5      Longevity (days)
                       =C5     =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
    jelly   extract    =C5  n  =C5  mean+/-S.D. =C5     maximal
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=
=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
        1:10(3)        =C5 12  =C5            lethal
                       =C5     =C5              =C5
        1:10(4)        =C5 11  =C5  18,2+/-1,8  =C5       28
                       =C5     =C5              =C5
        1:10(5)        =C5 12  =C5  19,2+/-2,2  =C5       32
                       =C5     =C5              =C5
        1:10(6)        =C5 12  =C5  19,3+/-1,7  =C5       28
                       =C5     =C5              =C5
        1:10(7)        =C5 12  =C5  15,8+/-2,2  =C5       28
                       =C5     =C5              =C5
        1:10(8)        =C5 11  =C5  20,6+/-3.1  =C5       32
                       =C5     =C5              =C5
        1:10(9)        =C5  8  =C5  12,3+/-3,5  =C5       34
                       =C5     =C5              =C5
        1:10(10)       =C5 12  =C5  17,5+/-2,3  =C5       31
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=
=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
Conclusion: If the extract from royal jelly was applied  to  C.
elegans  during the whole life span in above described conditi-
ons,  the most effective concentration was 1:10(8).  It  seemed
for  me more=F67 =F60reasonable to study the effect on longevity of any
concentrations near 1:10(8).

Acknowledgment: The author wishes to express his thanks to  CGC
for providing C. elegans (Bristol, N2) and E. coli OP50.



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