From owner-dros@hgmp.mrc.ac.uk Mon Aug 16 16:03:24 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 331337D191 for ; Mon, 16 Aug 2004 16:03:24 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 869E27D195 for ; Mon, 16 Aug 2004 16:03:23 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 3CA9F7D191; Mon, 16 Aug 2004 16:03:22 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 92BE07D116 for ; Fri, 13 Aug 2004 22:00:59 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 9F57C7D0C7; Fri, 13 Aug 2004 22:00:57 +0100 (BST) To: bionet-drosophila@net.bio.net From: sa08383@odin.mdacc.tmc.edu (Kathleen Gajewski) Newsgroups: bionet.drosophila Subject: looking for protocol Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: <200408132100.i7DL0p20000290672@odin.mdacc.tmc.edu> MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 97DE27D116 for ; Fri, 13 Aug 2004 22:00:56 +0100 (BST) X-Received: from odin.mdacc.tmc.edu (odin.mdacc.tmc.edu [143.111.81.144]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 22F6A7D0C7 for ; Fri, 13 Aug 2004 22:00:54 +0100 (BST) X-Received: by odin.mdacc.tmc.edu (8.11.1/1.1.31.1/07Aug04-0129PM) id i7DL0p20000290672; Fri, 13 Aug 2004 16:00:51 -0500 (CDT) X-To: dros@net.bio.net (bionet Drosophila) X-Mailer: ELM [version 2.5 PL5] Date: Mon, 16 Aug 2004 16:03:22 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Do any of you fly people have a good protocol for blue lacZ staining to paraffin sections? I've tried staining whole thoraces (after removing the head and abdomen) but I get a lot of false stain where I've made the cuts. Anyone have any solutions? Thanks KG --- .