From owner-drosophila@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!news From: Jens Riese Newsgroups: bionet.drosophila Subject: antisense transformants? Date: 25 Oct 1995 15:48:56 GMT Organization: MRC Laboratory of Molecular Biology Lines: 9 Message-ID: <46lm98$bn5@lyra.csx.cam.ac.uk> NNTP-Posting-Host: macs29-1.mrc-lmb.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.drosophila Has anybody tried making antisense expressing transformants? I am thinking of interfering with the function of a gene for which there are no mutants, and this is a possibility I am considering. I would appreciate hearing about any kind of experiences, positive or negative, to evaluate the feasibility of this approach. Thanks, Jens .