From owner-biophysics@net.bio.net Sun Jan 18 22:00:00 1998
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From: David Scott <djs17@york.ac.uk>
Newsgroups: bionet.biophysics
Subject: Quadratic binding equation
Date: Mon, 19 Jan 1998 12:00:31 -0800
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Hia
Here's one for all you all:
Binding of a ligand can be characterised by the hyperbolic binding curve
as we all know. However, under certain circumstances, we tend to use the
quadratic binding equation, or tight binding relationship:	
		v=(-(Kd+t+Cap)+/- sqr((Kd+t+Cap)^2+4.t.Cap))/2
where v is amount bound, t is total ligand conc, Cap is total binding
capacity and Kd is dissn constant.
 Now I've been happily using this for years for the appropriate cases,
but where does it come from and who derived it first?
 
Dave.

.