From owner-biotechniques@net.bio.net Thu Nov 30 22:00:00 1995
Path: biosci!biosci!not-for-mail
From: bkodrzyc@awod.com (Bob Kodrzycki)
Newsgroups: bionet.journals.letters.biotechniques
Subject: Re: UV inactivation of DNA on surfaces
Date: 1 Dec 1995 10:55:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <199512011705.MAA22518@sumter.awod.com>
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>I have been trying to find references as to the efficacy of using 
>ultraviolet light to reduce/eliminate potential contaminating template in 
>a PCR area.  The references I have found in "Biotechniques" publication 
>and others deal with DNA in solution but not on surfaces.  I am 
>interested in the UV source, intensity and duration.  If this is an 
>inappropriate forum for such a question please advise.
>
>					Sincerely,
>					William Weaver
>
>
>
We have successfully used a bacterial hood equipt with a UV light to
decontaminate surfaces
and eliminate contamination in PCR reactions.  I'm not sure what intensity
etc., but this is a standard bacterial benchtop unit.  Similar units are
available from Fisher or Baxter.  Also, I've seen "PCR" hoods advertised but
I'm not sure of the manufacturer.

Bob K
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  Bob Kodrzycki                 bkodrzyc@awod.com
  Westvaco Forest Research      (803) 851-4775
  Summerville, SC 29484         (803) 875-7185 FAX

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