From owner-drosophila@net.bio.net Mon Feb 06 22:00:00 1995
Path: biosci!RASCAL.MED.HARVARD.EDU!eberl
From: eberl@RASCAL.MED.HARVARD.EDU
Newsgroups: bionet.drosophila
Subject: mounting medium
Date: 6 Feb 1995 17:10:31 -0800
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Re: alternative mounting medium for Hoyer's.

Dear Alan,
I have used Ed Lewis' formula for mounting embryonic or larval cuticles
(Nature 276:565-570 (1978)).  It is very simple (9 parts 85% lactic acid, 1
part 95% ethanol) and gives adequate clearing to see all cuticular
structures after incubating on a 50 degC slide warmer for 12-24 hr.
Good luck,
Dan.



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Daniel F. Eberl                   |
Department of Genetics            |
452 Warren Alpert Building        |
Harvard Medical School            |
200 Longwood Avenue               |
Boston, MA 02115-6092             |
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