From owner-biotechniques@net.bio.net Thu Aug 14 23:00:00 1997
Path: biosci!biosci!not-for-mail
From: MMCCARTHY@biotechnet.com
Newsgroups: bionet.journals.letters.biotechniques
Subject: BioTechniques 23(3), September 1997
Date: 15 Aug 1997 05:25:10 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 191
Sender: daemon@net.bio.net
Approved: MMCCARTHY@BIOTECHNET.COM
Distribution: world
Message-ID: <01IMFN1JNWF68ZHC2W@delphi.com>
NNTP-Posting-Host: net.bio.net

BioTechniques 23(3), September 1997
Table of Contents

Benchmarks

Automated Fluorescence-Based Screening for
Mutation by SSCP: Use of Universal M13 Dye
Primers for Labeling and Detection
     P. Boutin, E.H. Hani, F. Vasseur, C.
     Roche, B. Bailleul, J. Hager and P.
     Froguel

DNA Sequencing with Modular Primers Using a
Two-Step Protocol with Thermostable
Polymerase at the Second Step
     M.C. Raja, D. Zevin-Sonkin, J.
     Shvartzburd, L. Kotler and L. Ulanovsky

Sequencing of 3-prime cDNA Clones Using
Anchored Oligo(dT) Primers
     J. Liao and Z. Gong

Post-PCR Labeling Using Taq DNA Polymerase
     Z. Chen and D.E. Ruffner

Identification of Targeted Embryonic Stem
Cells Using Long-Distance PCR
     E.V. Loukianov, T. Loukianova and M.
     Periasamy 

Enhancement of PCR-RFLP Typing of Bovine
Leukocyte Adhesion Deficiency
     A. Zsolnai and L. Fesus

Directional Immobilization of Heparin onto
the Nonporous Surface of Polystyrene
Microplates
     V.D. Nadkarni and R.J. Linhardt

Enhancement of Diaminobenzidine Colorimetric
Signal in Immunoblotting
     L.A. Pukac, J.E. Carter, K.S. Morrison
     and M.J. Karnovsky  

Even Highly Homologous Proteins Vary in their
Affinity for Different Blotting Membranes
     N. Balasubramanian and S.M. Zingde

Useful Vectors for the Two-Hybrid System in
Mammalian Cells
     M. Buchert, S. Schneider, M.T. Adams,
     H.P. Hefti, K. Moelling and C.M. Hovens

Simultaneous Expression of Multi-Subunit
Proteins in Mammalian Cells Using a
Convenient Set of Mammalian Cell Expression
Vectors
     M.I. Cockett, R. Ochalski, K. Benwell,
     R. Franco and J. Wardwell-Swanson

Site-Directed Mutagenesis: A Two-Step Method
Using PCR and DpnI
     S. Li and M.F. Wilkinson

PCR-Based Random Mutagenesis Using Manganese
and Reduced dNTP Concentration
     J.L. Lin-Goerke, D.J. Robbins and J.D.
     Burczak

Near-Zero Background Cloning of PCR Products
     Q. Zeng, M.K. Eidsness and A.O. Summers

Ligation of 5-prime DNA Overhangs to 3-prime
DNA Overhangs Using Short Single-Stranded
Oligonucleotide Linkers
     G. Zhao and M. Hortsch

Nuclei Isolation from Bone Cells for Nuclear
Run-on Assays
     N. Elango, J. Vivekananda, R. Strong and
     M.S. Katz 

Ammonium Acetate Protocol for the Preparation
of Plasmid DNA Suitable for Mammalian Cell
Transfections
     S.M. Saporito-Irwin, R.T. Geist and D.H.
     Gutmann

Rapid Purification of Reporter Group-Tagged
Inositol Hexakisphosphate on Ion-Exchange
Membrane Adsorbers
     A. Chaudhary, B. Mehrotra and G.D.
     Prestwich

Modified Semliki Forest Virus Expression
Vector that Facilitates Cloning
     J. Meanger, I. Peroulis and J. Mills    

Set of Optimized Luciferase Reporter Gene
Plasmids Compatible with Widely Used CAT
Vectors
     J. Altschmied and J. Duschl


The Internet On-Ramp
     Cyberspace for Biologists



Short Technical Reports

Quantification of Transcript-to-Template
Ratios as a Measure of Gene Expression Using
RT-PCR
     I. Harting and R.J. Wiesner

Design and Testing of beta-Actin Primers for
RT-PCR that Do Not Co-amplify Processed
Pseudogenes
     T. Raff, M. van der Giet, D. Endemann,
     T. Wiederholt and M. Paul

Use of Two Reverse Transcriptases Eliminates
False-Positive Results in Differential
Display
     Y.-J. Sung and R.B. Denman    

High-Stringency Subtraction for the
Identification of Differentially
Regulated cDNA Clones
     C.P. Scutt and P.M. Gilmartin 

Measurement of Telomeric DNA Content in Human
Tissues
     J.E. Bryant, K.G. Hutchings, R.K. Moyzis
     and J.K. Griffith

Controlled Gene Gun Delivery and Expression
of DNA Within the Cornea
     D.L. Tanelian, M.A. Barry, S.A.
     Johnston, T. Le and G. Smith  

Phosphorylation-Directed Antibodies in
High-Flux Screens for Compounds that Modulate
Signal Transduction
     J.A. Alberta and C.D. Stiles

One Primer Pair Amplifies Small Subunit
Ribosomal DNA from Mitochondria, Plastids and
Bacteria
     P. Berschick

Analysis of Assembly of Synthetic Antibody
Fragments: Expression of Functional scFv with
Predefined Specificity
     N. Kobayashi, E. Soderlind and C.A.K.
     Borrebaeck


Research Reports

Multiplex PCR: Critical Parameters and
Step-by-Step Protocol
     O. Henegariu, N.A. Heerema, S.R. Dlouhy,
     G.H. Vance and P.H. Vogt

PNA-Mediated Purification of PCR Amplifiable
Human Genomic DNA from Whole Blood
     C. Seeger, H.-G. Batz and H. Orum  

In-Cell Assembly of scFv from Human Thyroid
Infiltrating B Cells
     N. Chapal, M. Bouanani, M.J. Embleton,
     I. Navarro-Teulon, 
     M. Biard-Piechaczyk, B. Pau and S.
     Peraldi-Roux


Product Application Focus

Early Detection of Apoptosis Using a
Fluorescent Conjugate of Annexin V
     G. Zhang, V. Gurtu, S.R. Kain and G. Yan

Fluorescent Dye Assay for Detection of DNA in
Recombinant Protein Products
     R. Bolger, F. Lenoch, E. Allen, B.
     Meiklejohn and T. Burke

New Products
Index to Advertisers

.