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Subject: BioTechniques 23(2), August 1997
Date: 26 Jul 1997 19:27:57 -0700
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BioTechniques 23(2), August 1997
Table of Contents

Benchmarks

In Situ RT-PCR Using Fluorescence-Labeled
Primers
     U. Stein, W. Walther, J. Wendt and T.A.
      Schild

Purification and Cloning of Differential
Display Products
     S. Gery and S. Lavi

PCR Synthesis of cDNA from Total RNA
     S.B. Shepard and A.G. Cooper

Pitfalls of PCR: Cross-Reactivity with
Joyride E. coli Nucleic Acid
     M. Kenzelmann and K. Muhlemann

Rapid Cloning of PCR-Derived RAPD Probes
     A.M. Comes, J.F. Humbert and F. Laurent

PCR-Based Multiplex Method for Rapid
Screening of Recombinant Bacteria
     S. Schillberg, D. Schumann and R.
     Fischer

Direct Sequencing of PCR-Amplified 23S rDNA
     A. Ibrahim and A. Sjostedt

Method to Reverse the Order of Multiple
Cloning Sites
     C.-S. Lin

Immediate Digestion of Fish Muscle Following
Field Collections Yields DNA Suitable for
RAPD Fingerprinting
     J.S. Nelson, C.W. Khiong, C.L. Ming and
     V.P.E. Phang

Isolation of DNA Suitable for PCR for Field
and Laboratory Work
     J. Altschmied, U. Hornung, I. Schlupp, 
     J. Gadau, R. Kolb and M. Schartl

Use of a Dental Amalgamator to Extract RNA
from the Gram-Positive Bacterium
Streptococcus agalactiae
     H.H. Yim and C.E. Rubens

Quality Control of Centrifugal Elutriation
for Studies of Cell Cycle Regulation
     M. Hengstschlager, O. Pusch, T.
     Soucek,E., Hengstschlager-Ottnad and G.
     Bernaschek

Nonselective URA3 Colony-Color Assay in Yeast
ade1 or ade2 Mutants
     Y.-s. Weng and J.A. Nickoloff

Rapid Generation of DNA Probes by
Amplification of Tandem Repeats
     G. Trendelenburg and C. Hanski

Pyrophosphate in the Binding Reaction
Increases the Sensitivity of Mobility Shift
Analysis
     C. Fenton and M. Raafat El-Gewely

Re-probing of Immunoblots after Storage for
More than a Decade
     S.-U. Gorr

Universal Template Plasmid for Introduction
of the Triple-HA Epitope Sequence into Cloned
Genes
     M.H. Sato and Y. Wada


The Internet On-Ramp
     Cyberspace for Biologists


Short Technical Reports

Photoactivation of Caged Compounds in Single
Living Cells: An Application to the Study of
Cell Locomotion
     A. Ishihara, K. Gee, S. Schwartz, K. 
     Jacobson and J. Lee

Automated Differential Display Using a
Fluorescently Labeled Universal Primer
     N.R. Smith, M. Aldersley, A. Li, A.S. 
     High, T.P. Moynihan, A.F. Markham and
     P.A. Robinson

Use of Internal Controls to Increase
Quantitative Capabilities of
the Ribonuclease Protection Assay
     M.J.J. Davis, C.S. Bailey and C.K. 
     Smith, II

Increased Informativeness of RAPD Analysis by
Detection of Microsatellite Motifs
     J. Ramser, K. Weising, V. Chikaleke and
     G. Kahl

Efficient Cloning Method that Selects the
Recombinant Clones
     E. Loukianov, T. Loukianova and M. 
     Periasamy

Identification of Endonuclease Activity in
HIV-1 gp120 Preparations Produced Using
Baculovirus Expression Systems
     A.N. Davidoff and B.V. Mendelow

Long-Range and Highly Sensitive DNase I
Footprinting by an Automated Infrared DNA
Sequencer
     M. Machida, H. Kamio and D. Sorensen


Research Reports

Generation of Large Libraries of Random
Mutants in Bacillus subtilis by PCR-Based
Plasmid Multimerization
     S. Shafikhani, R.A. Siegel, E. Ferrari 
     and V. Schellenberger


Automated Cycle Sequencing with Taquenase:
Protocols for Internal
Labeling, Dye Primer and "Doublex"
Simultaneous Sequencing
     H. Voss, U. Nentwich, S. Duthie, S. 
     Wiemann, V. Benes, J.
     Zimmermann and W. Ansorge



Product Application Focus

Self-Seal Reagent: Evaporation Control for
Molecular Histology Procedures without
Chambers, Clips or Fingernail Polish
     D.E. Sullivan, L.E. Bobroski, O. Bagasra
     and M. Finney

New Products
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