From owner-celegans@net.bio.net Sun Jun 09 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.ksu.ksu.edu!news.cis.okstate.edu!usenet From: Ron Tate Newsgroups: bionet.molbio.proteins,bionet.celegans Subject: Re: help with a biochemistry lab Date: Mon, 10 Jun 1996 16:19:07 -0500 Organization: Oklahoma State University, Biochemistry and Molecular Biology Lines: 27 Message-ID: <31BC914B.6A3@bmb-fs1.biochem.okstate.edu> References: NNTP-Posting-Host: firefly3.biochem.okstate.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win95; I) Xref: biosci bionet.molbio.proteins:8092 bionet.celegans:935 Bruce Wightman wrote: > > I was wondering if anyone could provide suggestions for good proteins to > partially purify/purify for an introductory biochemistry lab for > undergraduates. I'd like to be able to give students an experience with > purification by gel filtration, ion exchange, and/or affinity > chromatography. Preferably, the protein should have an activity that can > be assayed easily and cheaply. The starting material should be something > that's clearly recognizable as "life". Unfortunately, the number of steps > has to be very limited, as time does not allow anything particularly > complicated. Also, it has to be doable at room temperature. > > Any ideas, good references, or protocols? How about lysozyme from egg white, its pretty easily purified by ion exchange and the assay is pretty easy too. Just another suggestion, its done here in an undergraduate lab course. -- >>>>>>---------------------------> >Ron Tate >Lab of Franklin Leach >Dept. of Biochem. & Molecular Biology >Oklahoma State University rtate@bmb-fs1.biochem.okstate.edu (405) 744-9326 --------------------------------------------- .