From owner-cellbiol@net.bio.net Tue Feb 18 22:00:00 1997
Path: biosci!MCHIP00.MED.NYU.EDU!alperd01
From: alperd01@MCHIP00.MED.NYU.EDU (Deborah Alpert)
Newsgroups: bionet.cellbiol
Subject: PARP cleavage and apoptosis
Date: 18 Feb 1997 21:27:46 -0800
Organization: NYU School of Medicine
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PARP (poly ADP ribose polymerase) cleavage is downstream of ICE-like
protease action in the induction of apoptosis. However, does PARP
cleavage (as demonstrated by Western blot) necessarily imply apoptosis
as the mechanism of cell death?  Does a time course demonstration of the
absence of PARP cleavage (spanning a period of time when cell death is
morphologically observed) exclude apoptosis?  Also, any tips on
detecting PARP cleavage, especially using the new anti-PARP antibodies
from Boehringer, would be greatly appreciated.

Thanks in advance,
Debbie Alpert
NYU School of Medicine

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