From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Hong- Gil Nam Newsgroups: bionet.diagnostics Subject: [Q} finding a mutation site on a BAC clone Date: 4 Dec 1996 07:24:55 -0800 Organization: POSTECH Lines: 20 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <584547$12e@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Netters: I am trying to clone a gene for gi gene in Arabidopsis. We have isolated a BAC clone (150kb) that are very likely to encompass the mutation. We would like to find the mutation site in the BAC clone. The BAC clone is form wild type and we have three independent alleles of mutants. I would appreciate if someone give an inforamtion on how to find a mutation site on a BAC clone in our situation? Hong Gil Hong Gil Nam Department of Life Science Pohang University of Science and Technology Hyoja Dong, Pohang Kyungbuk, 79-784 South Korea .