From owner-drosophila@net.bio.net Sun Oct 22 22:00:00 1995
Path: biosci!UTSW.SWMED.EDU!clark02
From: clark02@UTSW.SWMED.EDU (Kathleen Clark)
Newsgroups: bionet.drosophila
Subject: nuclear extracts
Date: 23 Oct 1995 14:34:03 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <01HWSBD9AQDG90ZQ2E@UTSW.SWMED.EDU>
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Dear Collegues,

   Has anyone tried to prepare nuclear extracts from Drosophila
ovaries/germ cells? It's my understanding that the nuclei are very fragile
and as such are hard to isolate intact. On the other hand, can anyone see a
problem with using unfractionated cellular protein for gel shift
experiments? Also, how much sucess have peple had at doing
immunolocalization on polytene chromosomes?  (i.e. looking at DNA binding
proteins)? Any feedback will be appreciated. 

Thanks!

Kathleen Clark
clark02@utsw.swmed.edu



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