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Subject: BioTechniques 23(5), November 1997
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BioTechniques 23(5), November 1997
Table of Contents

BENCHMARKS
                           
Preparative Electrophoresis: An Improved
Method for the Isolation of Human Recombinant
Apolipoprotein A-I
     H.H.-J. Schmidt, J. Genschel, R. Haas
     and M.P. Manns

Method to Reduce the Quantity of Ethidium
Bromide Required to Stain DNA in Agarose Gels
     M.J. Lucey, S.M. McColl and F.C.R.
     Manning

Simplification of Titer Determination for
Recombinant Baculovirus by Green Fluorescent
Protein Marker
     H.J. Cha, T. Gotoh and W.E. Bentley

Serum-Free Production, Concentration and
Purification of Recombinant Retroviruses
     J. Seppen, R.J. Kimmel and W.R.A.
     Osborne   

Semiquantitative Comparison of the
DNA-Binding Activity of In Vitro-Synthesized
Proteins
     P. Chene

Using a Eukaryotic GST Fusion Vector for
Proteins Difficult to Express in E. coli
     R.Y.L. Tsai and R.R. Reed

Production and Refolding of Recombinant
Leptin
     P.L. Zamorano, L. De Sevilla, V.B.
     Mahesh and D.W. Brann    

Manipulation of Transcription Factor Activity
Using Fluorescein-Tagged Dumbbell
Oligonucleotides
     M. Steinmayr, J.-P. Aubry and M.
     Becker-Andre

Cosmid Vector for the Cloning and Study of
Complex GC-Rich Genomes
     S. Ananvoranich and C. Simard

Duplication of a Region in the Multiple
Cloning Site of a Plasmid Vector to Enhance
Cloning-Mediated Addition of Restriction
Sites to a DNA Fragment
     L. Cocea

Stable DNA-Binding Yeast Vector Allowing
High-Bait Expression for Use in the
Two-Hybrid System
     O. Louvet, F. Doignon and M. Crouzet

High-Efficiency T-Vector Cloning of PCR
Products by Forced A-Tagging and
Post-Ligation Restriction Enzyme Digestion
     H. Bielefeldt-Ohmann and D.R.
     Fitzpatrick

PCR-Generated Crossover Linkers for
Site-Directed Mutagenesis
     A.C. Boyd and D.J. Porteous

Site-Specific Mutagenesis of Immunoglobulin
Domains by Multiple-Fragment Homologous
Recombination
     M. Eghtedarzadeh-Kondri, M.A. Walls and
     S.M. Glaser

In a Blinded Analysis, Restriction
Endonuclease Fingerprinting Detects All the
Mutations in a 1.9-kb Segment
     Q. Liu, J. Feng and S.S. Sommer

Nonradioactive Method for the Determination
of Internucleosomal Cleavage Associated with
Apoptosis
     C.-F. Wu, N.H. Bishopric and R.E. Pratt

Reliable Detection of DNA CpG Methylation
Profiles by the Isoschizomers MspI/HpaII
Using Oligonucleotide Stimulators
     D. Kupper, M. Reuter, A. Meisel and D.H.
     Kruger


THE INTERNET ON-RAMP
     Cyberspace for Biologists


SHORT TECHNICAL REPORTS

Cloning and Assembly of PCR Products Using
Modified Primers and DNA Repair Enzymes
     D.E. Watson and G.N. Bennett

Application of a Chimeric Green Fluorescent
Protein to Study Protein-Protein Interactions
     N. Garamszegi, Z.P. Garamszegi, M.S.
     Rogers, S.J. DeMarco and E.E. Strehler

Dicistronic LacZ and Alkaline Phosphatase
Reporter Constructs Permit Simultaneous
Histological Analysis of Expression from 
Multiple Transgenes
     X. Li, W. Wang and T. Lufkin

Detection of False-Negative Results in Nested
Primer PCR of Proviral HIV-1 DNA
     K. Zimmermann, B. Plaimauer, D. Schogl
     and J.W. Mannhalter 

Direct PCR of Small Genomic DNA Fragments
from Serum
     A.J. Sandford and P.D. Pare

Photoimmunodetection: A Nonradioactive
Labeling and Detection Method for DNA
     O.J. Marvik, M.L. Isaksen, L. Roza and
     B.H. Lindqvist 

Quantitation of In Vitro Activity of
Synthetic Trans-Acting Ribozymes Using HPLC
     L. Citti, L. Boldrini, S. Nevischi, L.
     Mariani and G. Rainaldi

Isolation of Full-Length RNA from a
Thermophilic Cyanobacterium
     X.-Z.J. Luo and S.E. Stevens, Jr.


BioFeature

Applications of Green Fluorescent Protein in
Plants
     S.M. Leffel, S.A. Mabon and C.N.
     Stewart, Jr.


RESEARCH REPORTS

Ribonuclease H Renaturation Gel Assay Using a
Fluorescent-Labeled Substrate
     L.-Y. Han, W.-P. Ma and R.J. Crouch

Focal Delivery of Neurotrophins into the
Central Nervous System Using Fluorescent
Latex Microspheres
     D.R. Riddle, L.C. Katz and D.C. Lo


PRODUCT APPLICATION FOCUS

Bifunctional lacZa-ccdB Genes for Selective
Cloning of PCR Products
     P. Gabant, P.-L. Dreze, T. Van Reeth, J.
     Szpirer and C. Szpirer

Infrared Fluorescent Detection of D1S80
Alleles from Blood and Body Fluid Collected
on IsoCode(TM) Devices
     R. Roy and L.R. Middendorf

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