From owner-celegans@net.bio.net Tue Nov 12 22:00:00 1996
Path: biosci!FRODO.MGH.HARVARD.EDU!hobert
From: hobert@FRODO.MGH.HARVARD.EDU (Oliver Hobert)
Newsgroups: bionet.celegans
Subject: fusion versus peptide antibody
Date: 13 Nov 1996 13:34:40 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199611132133.NAA11816@net.bio.net>
NNTP-Posting-Host: net.bio.net

To every worm-breeder who ever made an antibody:

We have contineous debates in our lab (based more on speculations than on
real facts) about which antibodies work better (or at all) on C.elegans
whole mount stains: antibodies raised against fusionproteins (GST, MBP
etc.) or antibodies raised against synthetic peptides.
Could everybody who ever made an antibody and tried it on worms send me a
note whether a anti-fusion antibody worked or didn't work or an
anti-peptide antibody worked or didn't work. I will assemble all answers
into a "statistic" and send it around.
Thanks all,

Oliver

-----------------------------------------------
Oliver Hobert
Harvard Medical School/Department of Genetics
Mass. General Hospital/Department of Mol. Biol.
Ruvkun-Lab
Wellman 8
Boston, MA 02114
Fax: 1-617-726 6893
Tel: 1-617-726 5983
e-mail: hobert@molbio.mgh.harvard.edu
-----------------------------------------------



.