From owner-celegans@hgmp.mrc.ac.uk Tue May 16 15:12:12 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4B65517B01; Tue, 16 May 2000 15:12:11 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id DCDB917AF2; Tue, 16 May 2000 15:12:09 +0100 (BST) To: celegans@net.bio.net Newsgroups: bionet.celegans Date: Tue, 16 May 2000 14:59:36 +0100 From: pmaciel@ibmc.up.pt (Patrícia Maciel) Subject: microinjection needles Organization: UnIGENe Message-Id: <20000516141209.DCDB917AF2@mercury.hgmp.mrc.ac.uk> Sender: owner-celegans@hgmp.mrc.ac.uk Precedence: bulk Dear Worm microinjectors: I´m a portuguese PhD student and I started my work in C. elegans last year. We bought a needle puller (Narishige PN-30) but we have some problems with needles. Can anyone with experience with this machine give me advice regarding the appropriate settings? thank you in advance Cláudia Santos UnIGENe/IBMC R. Campo Alegre, 823 4150 Porto Portugal csantos@ibmc.up.pt tel: +351-22-6074942 fax: +351-22-6099157 Content-type: text/x-vcard; name=pmaciel.vcf; charset=us-ascii Content-description: Card for PatrÌcia Maciel Content-disposition: attachment; filename=pmaciel.vcf Content-transfer-encoding: 7BIT Attachment converted: Morgan's Office iMac:pmaciel.vcf (TEXT/ttxt) (00010628) .