From owner-drosophila@net.bio.net Fri Jul 19 23:00:00 1996 Path: biosci!hawaii.edu!hampton From: hampton@hawaii.edu (Hampton Carson) Newsgroups: bionet.drosophila Subject: Re: eugracilis culture Date: 19 Jul 1996 19:10:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <4slr3i$4ac@taco.cc.ncsu.edu> NNTP-Posting-Host: net.bio.net Dear Mike: When I was collecting in New Guinea in 1977, I tried to rear all the wild species that I could: always using single, wild-caught females (isofemale lines> as the source so as not to get mixed cultures of sibling species or something like that. I noted that eugracilis always seemed to give only a few offspring: seemingly the females were only laying a few eggs, so that moulds in the food seemed to smother the few larvae there were present. Using your lab culture, I would try rearing from masses of 10-20 females in a small vial so the larvae produced can keep up with the mold.I had good luck rearing D. elegans, also an unusual melanogaster group species, that lays eggs only sparsely. We did find its breeding site: it oviposits in flowers of the morning-glory genus Ipomea. Many of these flower flies have this habit. Who knows! maybe eugracilis has this ecological niche! With best regards, Hamp Carson University of Hawaii at Manoa On Thu, 18 Jul 1996, Charles M Griswold wrote: > Could someone here give me some tips on eugracilis culture? > They are not doing well on our standard medium at 25C. > Thanks, Mike > -- > Mike Griswold grswld@unity.ncsu.edu > North Carolina State University voice: 919-515-5815 > Department of Genetics FAX: 919-515-3355 > Campus Box 7614 > Raleigh, NC 27695 > > > .