From owner-celegans@net.bio.net Mon May 24 15:29:00 1999 Path: biosci!biosci!not-for-mail From: Cristina Lagido Newsgroups: bionet.celegans Subject: Maximum density in liquid cultures of C. elegans Date: 24 May 1999 09:29:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <7ibupl$7bk@net.bio.net> NNTP-Posting-Host: net.bio.net Subject: Maximum density in liquid cultures of C. elegans Dear all, can anyone help me: what is the maximum density of L1 worms that can be inoculated into S medium + OP50 (30-40 g/l), incubated at 25C, that will give rise to adults and not dauers? Cheers Cristina Lagido University of Aberdeen Scotland .