From owner-dros@hgmp.mrc.ac.uk Thu Apr 5 15:17:58 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DA2DD17B04 for ; Thu, 5 Apr 2001 15:17:55 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E878817B90 for ; Thu, 5 Apr 2001 15:17:46 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id A863D17AA4; Thu, 5 Apr 2001 15:17:38 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D6CEF17C39 for ; Thu, 5 Apr 2001 09:44:26 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 75A7F17A72; Thu, 5 Apr 2001 09:44:13 +0100 (BST) To: bionet-drosophila@net.bio.net From: s.chyb@ic.ac.uk (Sylwester Chyb) Newsgroups: bionet.drosophila Subject: transfecting S2 cells Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BF4B317C4B for ; Thu, 5 Apr 2001 09:44:07 +0100 (BST) X-Received: from bammbamm.wye.ac.uk (bammbamm.wye.ac.uk [195.194.231.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B296617A99 for ; Thu, 5 Apr 2001 09:43:49 +0100 (BST) X-Received: from [195.194.230.19] (helo=icex10.wye.ic.ac.uk) by bammbamm.wye.ac.uk with esmtp (Exim 3.12 #1) id 14l5Mw-0004HF-00 for dros@net.bio.net; Thu, 05 Apr 2001 09:43:54 +0100 X-Received: from [195.194.227.50] (dhcp227050.wye.ac.uk [195.194.227.50]) by icex10.wye.ic.ac.uk with SMTP (Microsoft Exchange Internet Mail Service Version 5.5.2653.13) id HJQ70BDA; Thu, 5 Apr 2001 09:43:48 +0100 X-Sender: ic\sbs99sc/s.chyb@icex10.wye.ic.ac.uk X-To: dros@net.bio.net Date: Thu, 5 Apr 2001 15:17:38 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Can anyone share their experiences transfecting S2 cells? Did anybody have much luck with electroporation and if so could share a protocol? Thanks, Sylwek -- Dr. Sylwester Chyb Biology Imperial College University of London Wye Campus Wye, Kent TN25 5AH United Kingdom phone +44 0207 594 2770 fax +44 0207 594 2640 mobile 0780 359 6038 s.chyb@ic.ac.uk --- .