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Subject: BioTechniques 23(4), October 1997
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BioTechniques 23(4), October 1997
Table of Contents

Benchmarks

Modification of a PCR-Based Site-Directed
Mutagenesis Method
     C.L. Fisher and G.K. Pei

Typing of Human Papillomaviruses by
Reductional RFLP Analysis of Biotin-Labeled
PCR Fragments
     S.-Y. Wang, W.-M. Liu, T.-S. Wang, H.-F.
     Chou and K.-B. Choo

Long-Term Storage of Unamplified Complete PCR
Mixtures
     U. Reischl, B. Haber, W. Vandezande, T.
     De Baere and M. Vaneechoutte

Preparation of DNA from Numerous Individual
Microscopic Organisms for PCR-Based Assays of
Environmental Samples
     J.L. Bell and J.P. Grassle

Site-Directed Mutagenesis: A Two-Step Method
Using PCR and DpnI
     S. Li and M.F. Wilkinson

Improved Alkaline Lysis Method for Rapid
Isolation of Plasmid DNA
     A. Zhou, X. Jiang and X. Xu

High-Yield Method for Isolation of Lambda DNA
     S.H. Lee and J.B. Clark

Efficient Recovery of Plasmid DNA from
Erwinia herbicola with High Nuclease Activity
     S. Koul, V. Verma, A. Kumar and G.N.
     Qazi

Rapid Preparation and Identification of
Insert-Containing Recombinant Plasmid DNA
     B. Li, K.Y. Pilcher, T.E. Wyman and C.A.
     Machida   

Microgranular Cellulose Improves dsRNA
Recovery from Plant Nucleic Acid Extracts
     Y.G. Choi and J.W. Randles

Design and Use of Easily Made RNA Size
Markers
     Y. Audic, F. Omilli, H.B. Osborne and L.
     Landais

RT-PCR Detection of RNA Viruses in Stool
Specimens
     M.A. Drebot and S.H.S Lee

Vector for Positive Selection of In-Frame
Genetic Sequences
     N.A. Hawke, S.J. Strong, R.N. Haire and
     G.W. Litman    

Baculoviral Transfer Vectors for Expression
of FLAG Fusion Proteins in Insect Cells
     S.S. Koh, C.J. Hengartner and R.A.Young

Simplified ELISA for Detecting Antibodies to
Recombinant Fusion Proteins
     S. Carmona, W. Prinz and P. Arbuthnot   

Generation of Moderate Amounts of Polyclonal
Antibodies in Mice
     W. Luo and S.-H. Lin

Hybridization and Detection of Biotinylated
Oligonucleotide Probes in Agarose Gels
     L.M. Jarrell and J.D. Diehl, Jr.   

Improved Electrophoretic Separation of
Polymorphic Short Tandem Repeats in Agarose
Gels Using bis-Benzimide
     V. Nedelcheva Kristensen and A.-L.
     Borrensen-Dale

High-Temperature Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
     J.R. Haeberle

Safranin O Counterstaining Enhances the
Counting of b-Galactosidase-Expressing Cells
     K.-C. Ho and P.-S. Lin

Improved Procedure for Electroporation of
Peptides into Adherent Cells In Situ
     K.L. Firth, H.L. Brownell and L. Raptis 

Simplified Agar Plate Method for Quantifying
Viable Bacteria
     B.D. Jett, K.L. Hatter, M.M. Huycke and
     M.S. Gilmore

Low-Voltage Electric-Discharge Biolistic
Device
     S. Takagi and M. Kimura

Modification of an In Situ Renaturation
Method for Analysis of Protein Kinase
Activity with Multiple Substrates
     T.C. Fox and M.E. Rumpho

Efficient 5 -End Labeling of Oligonucleotides
Containing Self-Complementary Sequences
     L. Quijada, D. Moreira, M. Soto, C.
     Alonso and J.M. Requena

Detection of Major Histocompatibility Complex
Class I Antigens on the Surface 
of a Single Murine Blastocyst by Immuno-PCR
     A.S. McElhinny and C.M. Warner     

Method to Improve Reliability of
Random-Amplified Polymorphic DNA Markers
     F.J. Gallego and I. Martinez  

Variable-Size Injectable Dialysis Chambers
     P.M. Debnam and D.H. Kohl

Ethidium Bromide Enhances Transformation of
E. coli with Homopurine/Pyrimidine-Rich DNA
     Y. Zhixing and J.-L. Nahon    


BioFeedback


The Internet On-Ramp
     Cyberspace for Biologists


Short Technical Reports

Expression of Green Fluorescent Protein in
Aureobasidium pullulans and Quantification of
the Fungus on Leaf Surfaces
     A.J. Vanden Wymelenberg, D. Cullen, R.N.
     Spear, B. Schoenike and J.H. Andrews    

nbgeo, A Combined Selection and Reporter Gene
for Retroviral and Transgenic Studies
     J. Blake, P.C. Salinas and S.M. Hughes  

Flow Cytometric Quantification of
Surface-Displayed Recombinant Receptors on
Staphylococci
     C. Andreoni, L. Goetsch, C. Libon, P.
     Samuelson, T.N. Nguyen, A. Robert, M.
     Uhlen, H. Binz and S. Stahl

PCR Mutagenesis-Based Method for Generation
of Positive Controls for SSCP Analysis
     A. Ruiz, G. Antinolo, S. Borrego, B.
     Sanchez and J. Sanchez

Competitive Oligonucleotide Single-Base
Extension Combined with Mass Spectrometric
Detection for Mutation Screening
     G.S. Higgins, D.P. Little and H. Koster
     
Specific Inhibition of PCR by Non-Extendable
Oligonucleotides Using a 5-prime to 3-prime
Exonuclease-Deficient DNA Polymerase
     D. Yu, M. Mukai, Q. Liu and C.R.
     Steinman

Calibration and Storage of DNA Competitors
Used for Contamination-Protected Competitive
PCR
     T. Kohler, A.-K. Rost and H. Remke 


Research Reports

Copper Treatment Increases Recombinant
Baculovirus Production and Polyhedrin and p10
Expression
     L.M. Lanier, K. Storm, A. Shafaie and
     L.E. Volkman

Studying Electric Field Effects on Embryonic
Myocytes
     A. Lazrak, G.D. Griffin and P.C. Gailey 


Product Application Focus

Multiplex Systems for the Amplification of
Short Tandem Repeat Loci: 
Evaluation of Laser Fluorescence Detection
     M.D. Ricciardone, A.M. Lins, J.W. Schumm
     and M.M. Holland

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