From owner-cellbiol@net.bio.net Sun Feb 16 22:00:00 1997
Path: biosci!NWU.EDU!r-brown
From: r-brown@NWU.EDU (Clark Brown)
Newsgroups: bionet.cellbiol
Subject: obtainng gadph clone
Date: 17 Feb 1997 15:38:18 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I am looking for a human gadph clone. I would like to find one that is
in a prokaryotic vector that has phage promoters (T7 or sp6) on either
side of the gene. I would like to use it to make sense transcripts. I
would prefer to have it in the T7 orientation. I intend to use it to
quantitate levels of messages in cells. 

I understand that this is a particuliarly good gene to normalize input
of rna in assays like rpa's or tagman assays. If you have information to
the contrary I would be interested in that also. I am currently using
b-actin, but I understand that this message fluctuates depending on the
treatment of the cells because it has a serum promoter.

I would appreciate help in obtaining the clone and any information
pertaining to the suitability of using gadph to normalize rna input in
assays used to quantitate levels of rna.

Thanks,

Clark Brown, Ph.D.
clark@ipserver.cac.nwu.edu

.