From owner-celegans@net.bio.net Fri Feb 12 22:00:00 1999
Path: biosci!biosci!not-for-mail
From: sarafi@BINAH.CC.BRANDEIS.EDU
Newsgroups: bionet.celegans
Subject: GFP antibody conditions
Date: 13 Feb 1999 08:40:09 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Hello!
I was wondering if anyone has successfully used the Clontech polyclonal
anti-GFP antibody on worms?  We have tried using the Ruvkun/Finney
protocol, but have not had any luck with it. Any suggestions?

Also, does anyone know fixation conditions for antibody staining which
will allow GFP in the strain to remain visible?

Thanks for the help!
Trina Sarafi
(Sengupta lab)



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