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From: "John Philo" <jphilo_nospam_@mailway.com>
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References: <7msniv9297.fsf@jasmin.iwr.uni-heidelberg.de>
Subject: Re: Diffusion of GDH
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I'd be very surprised if you could find any experimental data for this. I
think you could safely assume the hydrodynamic radius of GDH will be fairly
constant, so if you can get the diffusion coefficient at normal temperatures
and aqueous conditions (or you could calculate that from the mass and a
sedimentation coefficient), you could then correct that value to your
conditions IF you can figure out the viscosity of this solvent at those
temperatures.

I know that viscosity data for D20 at 0-100 C is hard to come by, let alone
for deuterated methanol or mixtures. I think your best hope would be that
one of the people who study enzyme kinetics at low temperatures might have
figured out these viscosities, since obviously the viscosity also strongly
affects the rates of second order reactions.

John Philo
Alliance Protein Laboratories


"Torsten Becker" <torsten.becker@jasmin.iwr.uni-heidelberg.de> wrote in
message news:7msniv9297.fsf@jasmin.iwr.uni-heidelberg.de...
> Hi,
> i would need to know the diffusion constant of Glutamate
> Dehydrogenase in a 70% v/v Methanol/Water (CD3OD/D2O) solvent
> for the range of 80-310 Kelvin.
> Does anybody know, where to look for such things?
>
> Thank you very much!
>
>
>


----------------------------------------------------------------------------
----


> Torsten Becker  Biocomputing Group
> IWR, Universitaet Heidelberg
> Im Neuenheimer Feld 368, D-69120 Heidelberg, Germany.
> Tel: +49 6221 54 8808
> torsten.becker@iwr.uni-heidelberg.de
>



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