From owner-celegans@net.bio.net Thu Feb 01 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!usenet.ins.cwru.edu!ukma!mik.uky.edu!mlgood00
From: mlgood00@mik.uky.edu (michael goodson)
Newsgroups: bionet.celegans
Subject: RT-PCR Control
Date: 2 Feb 96 14:42:26 GMT
Organization: University of Kentucky, Dept. of Math Sciences
Lines: 26
Message-ID: <mlgood00.823272146@mik.uky.edu>
NNTP-Posting-Host: nx22.mik.uky.edu
Keywords: RT-PCR, cDNA, mRNA

I am going to start doing some RT-PCR (reverse transcrition coupled with
polymerase chain reaction).  I am trying to decided on an mRNA message
that I can use as an internal control.  The ideal candidate would be a
message that meets the following criteria. . .

   1.  Known cDNA sequence.
   2.  Ubiquitously expressed during all stages of development.
   3.  Expressed at moderate to low levels.  

I found RNA Polymerase II, but I am afraid that its levels may be too
high to use.  I am looking for a message which could be expressed at low
levels during only a couple of stages of development. 

If anyone knows of a suitable message to use, please let me know (either
by e-mail, preferred, or by reply posting to this group).

Thanks,
Michael

________________________________________________________________________________
         Michael Goodson              |   Department of Biochemistry   
      Phone: (606) 257-7349           |     University of Kentucky
       Fax: (606) 323-1037            |          800 Rose St.
   E-mail: goodson@pop.uky.edu        |    Lexington, KY 40536-0084  
________________________________________________________________________________


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