From owner-drosophila@net.bio.net Wed Sep 07 23:00:00 1994 Newsgroups: bionet.drosophila Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!umn.edu!dialup-1-71.gw.umn.edu!user From: kirs0012@gold.tc.umn.edu (Andrew Kirscher) Subject: PCR RAPD's w/Single Fly extractions Message-ID: Followup-To: bionet.drosophila Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: dialup-1-71.gw.umn.edu Organization: University of Minnesota Date: Thu, 8 Sep 1994 20:51:16 GMT Lines: 8 I am currently working on a project to show genetic differences using RAPD markers in a population genetics lab at the University of Minnesota. I am looking for possible additional protocols for single fly extractions for use with PCR. I am currently using "Engel's" method, which is working pretty well, but am having some problems still. Any suggestions? Any new PCR recipes? Thanks Andrew W. Kirscher. .