From owner-drosophila@net.bio.net Wed Apr 05 23:00:00 1995 Newsgroups: bionet.drosophila Path: biosci!daresbury!trane.uninett.no!nntp.uio.no!nac.no!Norway.EU.net!EU.net!howland.reston.ans.net!torn!utnut!erin.utoronto.ca!tuzo.erin!nwinegar From: nwinegar@tuzo.erin (Neil Winegarden) Subject: Re: SL2 cells at 37 Message-ID: Sender: news@credit.erin.utoronto.ca (Usenet News) Nntp-Posting-Host: tuzo.erin Reply-To: nwinegar@tuzo.erin.utoronto.ca Organization: Erindale College, University of Toronto, Canada References: <1995Apr5.141110.4497@alw.nih.gov> Date: Wed, 5 Apr 1995 23:02:16 GMT Lines: 19 First off, there is a perfect person to ask at NIH. Talk to Dr. Carl Wu in the laboratory of Biochemistry NCI. He studies Heat shock factor in Drosophila and could give some useful insight for your situation. In addition, my experience would suggest that perhaps using 36 or even 35 C (for 15 to 20 minutes) would be more useful. You will still get transcription from a heat shock promoter, and your cells will recover faster. Hope that this is of some help, Cheers -- ________________________________ Neil Winegarden University Of Toronto nwinegar@credit.erin.utoronto.ca .