From owner-celegans@net.bio.net Thu Jul 14 23:00:00 1994 Path: biosci!PHOENIX.PRINCETON.EDU!yuan From: yuan@PHOENIX.PRINCETON.EDU ("Jeffrey Yuan") Newsgroups: bionet.celegans Subject: Re: Specific Knockouts in C.elegans Date: 15 Jul 1994 07:33:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <199407151427.AA26081@ponyexpress.princeton.edu> Reply-To: "Jeffrey Yuan" NNTP-Posting-Host: net.bio.net In message <01HEQ9BKHG8Y8WWXWH@acs.wooster.edu> William R. Morgan writes: > smh1008@cus.cam.ac.uk (David S. Huen) writes: > > >Is it possible to produce mutants in C.elegans in which a specific gene is > >>knocked out? > > Yes, see Zwaal et al. (1993) Target-selected gene inactivation in C. > elegans by using a frozen transposon insertion mutant bank. Proc. Natl. > Acad. Sci. USA 90: 7431-7435. > > Bill Morgan > > William R. Morgan > The College of Wooster > Department of Biology > 931 College St. > Wooster, OH 44691 > Phone: 216-263-2026 > FAX: 216-263-2378 > E-mail: wmorgan@acs.wooster.edu > > > You may also contact Ron Plasterk (the senior author on the Zwaal paper) in the Netherlands. He wrote a chapter for the upcoming C.elegans Methods Book called "Reverse Genetics: from Gene Sequence to Mutant Worm." This is a detailed description of the process and how to do it. He may be able to send you a preprint of the chapter. His email address is rplas@nki.nl. I hope he does not get upset with me by posting this message. Jeffrey Yuan ************************************************************************** * Jeffrey Yuan * * Department of Molecular Biology * * Lewis Thomas Laboratory * * Princeton University FAX: 609-258-3980 * * Washington Road Phone: 609-258-5939 * * Princeton, NJ 08544 email: yuan@phoenix.princeton.edu * ************************************************************************** .