From owner-drosophila@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!emory!cssun.mathcs.emory.edu!hobbes.cc.uga.edu!news From: bernhardt@bscr.uga.edu Newsgroups: bionet.drosophila Subject: Re: Virgin collection Date: 17 Oct 1995 13:26:31 GMT Organization: University of Georgia, Athens, Ga Lines: 16 Message-ID: <460au7$9h7@hobbes.cc.uga.edu> References: <45f4h4$8ds@saba.info.ucla.edu>,<45nito$t9e@hobbes.cc.uga.edu> Reply-To: bernhardt@bscr.uga.edu NNTP-Posting-Host: bscr.cc.uga.edu In article <45nito$t9e@hobbes.cc.uga.edu>, bernhardt@bscr.uga.edu writes: >In article <45f4h4$8ds@saba.info.ucla.edu>, lscclab@biovx1.biology.ucla.edu (LSCCLAB) writes: >>Is there anything that can be done to increase the amount of virgins >>collected from the bottle > Collect often enough to prevent mating. Once every 12 hours >should do it. Note that this is a rule of thumb only. Temperature and species may require different counting intervals. One way to be sure you're getting virgins is to keep the culture bottles that you hold the alleged virgins around for a few days after you use them. If you see any larvae running around, the flies weren't all virgins, or you put a male in there. Ooops! -- Matt Bernhardt University of Georgia This space left unintentionally blank. bernhardt@bscr.uga.edu .