From owner-celegans@hgmp.mrc.ac.uk  Tue Feb 15 02:34:02 2000
Return-Path: <owner-celegans@hgmp.mrc.ac.uk>
Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110)
	id 53B9A17B1E; Tue, 15 Feb 2000 02:34:01 +0000 (GMT)
Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1)
	id 0147E17B1B; Tue, 15 Feb 2000 02:33:58 +0000 (GMT)
To: celegans@net.bio.net
Newsgroups: bionet.celegans
Date: Mon, 14 Feb 2000 15:04:14 +0200
From: gidi <bishemer@techunix.technion.ac.il>
Subject: mapk
Organization: Technion, Israel Institute of Technology
Message-Id: <20000215023358.0147E17B1B@mercury.hgmp.mrc.ac.uk>
Sender: owner-celegans@hgmp.mrc.ac.uk
Precedence: bulk

I'm trying to stain worms with anibodies against mh27 (adherens
junctions) and map kinase 1. They both origin from mouse so i have a
hard time defining between them. I have an mh27-gfp strain but I haven't
succeeded in ataining against mpk-1 without damaging the gfp-staining.
Is one of you good people know papers containing mapk antibody fixation
and staining and if anyone has tried doing it on a gfp background?





.