From owner-cellbiol@net.bio.net Mon Dec 09 22:00:00 1996 Path: biosci!agate!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.cellbiol Subject: Re: transformation or transfection? Date: Tue, 10 Dec 1996 20:08:55 +0000 Organization: University of Reading, U.K. Lines: 40 Message-ID: References: NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: On 6 Dec 1996 dlombard@[157.92.77.1] wrote: > > To: cellbiol@net.bio.net > > From: BehariRN@perkin-elmer.com ("BehariRN") > > Subject: Re: transformation or transfection? > > Date: 5 Dec 1996 11:55:11 -0800 > > > > > > > > > > > What is the difference between transfection and transformation of host cells by > > recombinant vector DNA containing a chimeric gene? Is the term transfection used > > for onlywhen the recombinant DNA is introduced into eukaryotic host cells and > > transformation for prokaryotic host cells > > > > Thanks > > > > > > > > I don't in accord with the transformatios term will be introduced > for prokaryotic cells. I think that transfection is the introduction > of foraneous DNA into cells (eukariotic or prokariotic) and > transformations is your consecueses. > > > Daniel M. Lombardo > Histologia y Embriologia > Facultad de Cs. Veterinarias. UBA Isn't it called transformation to put foreign DNA into a cell, pro- or eukaryotic, and transfection the particular case when the transformation is achieved by means of a viral vector? Also, I've heard of transformation achieved using Agrobacterium referred as trasfection. Nacho de las Heras Botany Dept. Reading University .