From owner-cellbiol@net.bio.net Mon Dec 09 22:00:00 1996
Path: biosci!daresbury!lyra.csx.cam.ac.uk!warwick!leicester!usenet
From: "Dr E. Buxbaum" <EB15@le.ac.uk>
Newsgroups: bionet.cellbiol
Subject: Re: protein sequencing.
Date: 10 Dec 1996 13:49:10 GMT
Organization: University of Leicester, UK (PCFS User)
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brackena@tcd.ie (Adrian Bracken) wrote:
>Is it possible to run samples on SDS gel and then cut out and sequence >the protein bands that are present in one lane and not in another? 

This is a standard technique: Separate by one- or twodimensional 
electrophoresis, western-blot onto PVDF membranes, stain with Coomassie 
blue or Ponceau red, cut out the interesting bands and put them (still on 
the PVDF) into a gas phase sequencer. 

You have to use PVDF membranes though, nitrocellulose does not work.


.