From owner-metabolic-reg@net.bio.net Tue Aug 03 23:55:00 1999 Newsgroups: bionet.metabolic-reg Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!newsfeed.icl.net!newspeer.clara.net!news.clara.net!easynet-tele!easynet.net!server5.netnews.ja.net!leeds.ac.uk!news From: bmbjmm@bmb.leeds.ac.uk (jez) Subject: Re: pre-steady state X-Accept-Language: en Message-ID: <37A789DA.F38C8E28@bmb.leeds.ac.uk> NNTP-Posting-Host: bmbpc250.leeds.ac.uk X-Mailer: Mozilla 4.5 [en] (WinNT; I) Content-Type: text/plain; charset=us-ascii Organization: university of leeds MIME-Version: 1.0 Date: Wed, 4 Aug 1999 01:23:12 +0100 (BST) References: <37A35B6A.47AAF3B7@bmb.leeds.ac.uk> <37A588BD.4840CA44@brookes.ac.uk> To: Mark Poolman Lines: 40 Content-Transfer-Encoding: 7bit Hi Mark My question is in reference to a number of kinetic studies e.g. hydrolysis of p-nirophenylphosphate (also check out a section in Fersht, Structure and Mechanism) monitoring of the pre-steady state in the above hydolysis reaction indicated that there was a rapid pre steady state (burst phase) and then a slow linear appearance of P when the steady state is attained. The slow step is attributed to the hydrolysis of the acyl enzyme intermediate Therefore, i was wondering if the information obtained from such studies is similar to that derived from monitoring single turnover reactions in which [E] >>[S] most of the data will be concerned with binding rates?? the software development sounds cool is this concerned with analysis? i am currently using origin for this but would not mind experimenting with other packages l8r, jez Mark Poolman wrote: > Jeremy Murray wrote: > > > I have a question concerning pre steady state kinetics wrt enzyme > > mechanism. > > What information can be obtained from such an analysis? > > If by pre steady state you mean analysis of time course data to extract > kinetic parameters, and/or compare canditate reaction mechanisms, I am > working on software that may be of some use. I would be interested in > hearing a bit more about it. > > Mark > > > .