From owner-celegans@net.bio.net Mon Oct 19 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Kim sun ja <sun@geguri.kjist.ac.kr>
Newsgroups: bionet.celegans
Subject: about b-galactosidase
Date: 20 Oct 1998 09:19:00 -0700
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Dear Worm Breeders,

We have worked with putitive ECM protein. The other day, I found that
secreted b-galactosidase could not function so lacZ construct that
contain transmembrane stop signal was used. Is it true? I have used NLS
containing LacZ promoterless vector. but If I microinjected new
construct  containing N-terminal secretary signal sequence, what will be
the expression pattern. I really appreciate if I got your reply.



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