From owner-drosophila@net.bio.net Thu Jul 04 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!nnrp.info.ucla.edu!news.ucdavis.edu!chip.ucdavis.edu!fznewt From: "Jeff \"Newt\" Sekelsky" Newsgroups: bionet.drosophila Subject: Re: Epitope tags Date: Fri, 5 Jul 1996 09:03:23 -0700 Organization: University of California, Davis Lines: 31 Message-ID: References: NNTP-Posting-Host: chip.ucdavis.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: fznewt@chip.ucdavis.edu In-Reply-To: Katy Afshar used FLAG to epitope tag NOD and demonstrate it's localization to meiotic chromosomes. She found that the M5 monoclonal antibody from Kodak (IB13091) worked well in Drosophila, with no cross-reacting bands on Western blots and no significant background flourescence when visualized by confocal microscopy. For details see: Afshar, K., Barton, N.R., Hawley, R.S. and Goldstein, L.S.B. (1995). DNA binding and meiotic chromosomal localization of the Drosophila Nod kinesin-like protein. Cell 81, 129-138. Afshar, K., Scholey, J. and Hawley, R.S. (1995). Identification of the chromosome localization domain of the Drosophila Nod kinesin-like protein. J.Cell Biol. 131, 833-843. -Jeff Sekelsky On Tue, 2 Jul 1996, Gerard Manning wrote: > I'm wish to epitope tag a protein for expression in S2 cells and in > embryos. I know several groups have used the myc tag, but also heard there > were problems with myc antibodies. Does anyone have experience (good or > bad) with myc, HA, flag or other epitope tags, and can reccomend the best > to use? > > Thanks in advance, > > Gerard Manning. > > .