From owner-cellbiol@net.bio.net Fri Feb 14 22:00:00 1997
Path: biosci!agate!newsgate.cuhk.edu.hk!news.cuhk.edu.hk!usenet
From: Rocky Ho <RockyHo@cuhk.edu.hk>
Newsgroups: bionet.cellbiol
Subject: ELISA against Quantitative Western Blot
Date: Sun, 16 Feb 1997 02:26:54 +0800
Organization: The Chinese University of Hong Kong
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Hi

Commerical ELISA kit I encounted is always stated to be used to detect
protein factors in the blood. If we want to quantify the amount of
certain cellular protein, tissue protein extraction and Western blot
must be carried out, and Chemiluminescence detection method then used to
quantify the amount of protein unit for different tissue sample.

Is it possible to use ELISA kit to replace the Quantitative Western Blot
method. For Western Blot, it is less common to use single monoclonal Ab,
while in ELISA, monoclonal clonal Ab is usually used to coat the plate.
So, will it be more specific to detect the quantity of specific protein
by ELISA method.

Rocky Ho

.