From owner-biotechniques@net.bio.net Thu Jan 21 22:00:00 1999
Path: biosci!biosci!not-for-mail
From: botao zhao <bzhao@uoft02.utoledo.edu>
Newsgroups: bionet.journals.letters.biotechniques
Subject: in vitro translation problems
Date: 22 Jan 1999 12:39:44 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Would like to know info abt in vitro translation of memebrane bound
proteins, eg  human muscarinic receptor..
have problems with translation, protein incorporates radioactivity, but
does not move into gel.. despite several attempts. is it due to
agregation of the protein molecules since there is no membrane present??
am curious.. 
pl.reply to vyjoo @hotmail.com
(am a doctoral student)



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