From owner-cellbiol@net.bio.net Thu Feb 13 22:00:00 1997
Path: biosci!BU.EDU!reddypvr
From: reddypvr@BU.EDU (padma reddy)
Newsgroups: bionet.cellbiol
Subject: use of slo for cell permeabilisation
Date: 14 Feb 1997 11:34:45 -0800
Organization: Boston Univ School of Medicine
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Sender: daemon@net.bio.net
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Message-ID: <3304BE2F.4C15@bu.edu>
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I have been trying to use SLO to selectively introduce pores in the 
plasma membrane of cells. I do this by incubating the cells with SLO  in 
an ice-water bath and then incubate the cells at 37 after washing the 
unbound SLO. However I find that the ER compartment is not really 
intact as I get a substantial amount of leakage. Can someone who has 
this system going give me some tips.
Thanks
Padma

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