From owner-celegans@net.bio.net Sun Jun 09 23:00:00 1996 Path: biosci!agate!garriga4.berkeley.edu!user From: wightman@mendel.berkeley.edu (Bruce Wightman) Newsgroups: bionet.molbio.proteins,bionet.celegans Subject: help with a biochemistry lab Date: Mon, 10 Jun 1996 10:26:47 -0800 Organization: UCBerkeley Lines: 18 Message-ID: Reply-To: wightman@mendel.berkeley.edu NNTP-Posting-Host: garriga4.berkeley.edu Xref: biosci bionet.molbio.proteins:8086 bionet.celegans:931 I was wondering if anyone could provide suggestions for good proteins to partially purify/purify for an introductory biochemistry lab for undergraduates. I'd like to be able to give students an experience with purification by gel filtration, ion exchange, and/or affinity chromatography. Preferably, the protein should have an activity that can be assayed easily and cheaply. The starting material should be something that's clearly recognizable as "life". Unfortunately, the number of steps has to be very limited, as time does not allow anything particularly complicated. Also, it has to be doable at room temperature. Any ideas, good references, or protocols? Thanks- Bruce Wightman Department of Molecular and Cell Biology 401 Barker Hall- Garriga Lab University of California Berkeley, CA 94720-3204 .