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From: justus.verhagen@psy.ox.ac.uk (Justus Verhagen)
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Subject: Re: Perforated Patch Problem
Date: Tue, 04 Sep 2001 09:22:47 GMT
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Alhtough not likely, but could your ampho have gone nearly completely
gone bad?


On 3 Sep 2001 20:27:23 -0700, mfireland@yahoo.com (Matthew Ireland)
wrote:

>Dear Colleagues 
>
>Currently I am using perforated patch on neurons within the CNS.
>Recently, I have been experinceing a problem when I try to perforate a
>neuron, instead of a full perforation as you would expect, it only
>results in a partial perforation.
>
>I have tried different concentrations of Amphotericin B, different
>filling times and modified electrodes. All without much success.
>
>I would appreciate any suggestions
>
>With thanks,
>
>Matthew Ireland
>University of Queensland


.