From owner-drosophila@net.bio.net Sun Nov 19 22:00:00 1995 Newsgroups: bionet.drosophila Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!news00.sunet.se!sunic!mn6.swip.net!news99.sunet.se!news.ki.se!drop-dead.cmb.ki.se!user From: christer.ericsson@mog.ki.se (Christer Ericsson) Subject: Re: coupling peptides for antibodies Sender: news@news.ki.se (News admin) Message-ID: Date: Mon, 20 Nov 1995 09:13:16 GMT References: Nntp-Posting-Host: drop-dead.cmb.ki.se Organization: Karolinska Institute Lines: 48 Cahir, I think the ³vertebrate people downstairs² give what would normaly be considered good advice, although I think there are numerous alternatives, such as BSA or OVA. I think you should affinity purify your positive antiserum, using your peptide conjugated to a carrier that is different from your immunogen, in order to get a truly monospecific (as monospecific as they get) antiserum. So, I think the carrier you use may not matter from a crossreactivity point of view. I hope this is helpful. Please contact me if there is anything else in this matter you would like to discuss. Sincerely Christer Ericsson In article , cok@mole.bio.cam.ac.uk (Cahir O'Kane) wrote: When raising antibodies to peptides, does anyone have any recommendations for or against particular proteins to which one can couple the peptide? The vertebrate people downstairs here recommend keyhole limpet hemocyanin - but given that this is also an arthropod, is there any potential problem with crossreactivity to a fly homolog of KLH? Thanks, Cahir O'Kane -- Cahir O'Kane, Dept of Genetics, Downing St., Cambridge, CB2 3EH, UK cok@mole.bio.cam.ac.uk -- "The best way to predict the future is to invent it" Alan Kay Christer Ericsson, PhD Assistant professor Dept. of Cell and Molecular Biology Medical Nobel Institute Karolinska Institute S-171 77 Stockholm Sweden Phone: +46 8 728 7358 Fax: +46 8 31 35 29 Videoconference: CU-SeeMe IP# 130.237.119.121 .