From owner-cellbiol@net.bio.net Thu Jan 16 22:00:00 1997
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From: Stephan Niland (100766.3150@compuserve.com) <100766.3150@CompuServe.COM>
Newsgroups: bionet.cellbiol
Subject: cell cycle synchronization
Date: 17 Jan 1997 22:20:08 GMT
Organization: CompuServe, Inc. (1-800-689-0736)
Lines: 13
Message-ID: <5botuo$883$1@mhadf.production.compuserve.com>

Hi, out there,
I´ve got three questions on cell culture synchronization:
1)how can I best synchronize a fibroblast cell culture? 
Is mitotic shake-off really the best, or are there any 
protocols using certain drugs? If yes, please mail me 
the respective reference or protocol!
2) How long will the culture probably be synchronous?
3) Are there possibilities to arrest such cultures in G1-,
 S-, G2-, or M-phase? if yes, how can I do it?
I would be glad, if you could help me, and I am looking 
forward to receiving your mail!

Stephan (e-mail: 100766.3150@compuserve.com)

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