From owner-drosophila@net.bio.net Sun Nov 19 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!torn!nott!uottawa!news From: dhickey@maeve.genetics.uottawa.ca Newsgroups: bionet.drosophila Subject: Re: luciferase in Drosophila Date: 20 Nov 1995 17:10:00 GMT Organization: University d'/of Ottawa Lines: 12 Message-ID: <48qcp8$p6m@mercury.cc.uottawa.ca> NNTP-Posting-Host: maeve.genetics.uottawa.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Joachim Urban writes : I was wondering if anyone has tried the luciferase system in Drosophila. We have made a series of transgenic lines in which the luciferase coding region is under the control of the alpha amylase promoter. These strains express high levels of luciferase activity. This activity is confined to the midgut and is repressible by dietary glucose, thus reflecting the activity pattern of the amylase promoter. This promoter may not be suitable for studies on early embryogenesis, but the system should work with any promoter. Donal Hickey. .