From owner-cellbiol@net.bio.net Thu Feb 13 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!EU.net!Norway.EU.net!nntp.uio.no!news.kth.se!newsfeed.sunet.se!news99.sunet.se!news.uni-c.dk!news-inn.uni-c.dk!news.uni-c.dk!not-for-mail From: Jens Rainer Hansen Newsgroups: bionet.cellbiol Subject: IL-4 assay on PBMC, need help ! Date: Fri, 14 Feb 1997 15:28:18 +0100 Organization: Leo Pharmaceutical Products Lines: 13 Message-ID: <33047682.4F10@leo.dk> NNTP-Posting-Host: balder.leo.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) HI' Does anyone have experience with an IL-4 stimulation assay, stimulating human PBMC with PHA, Protein A, CD2+CD28 antibody etc. I have tried it all for different incubation times and conc. I get max about 100-300 pg/ml IL-4, using a final conc. of 1x10**6 cells/ml. I know that only a fraction of the cells are producing IL4. I have not via the litterature seen others who got more IL4 produced. So perhaps it is essentially not possible to get more IL4 produced. It would be a great help if somebody could give me a lead. Thanks Jens Rainer .