From owner-drosophila@net.bio.net Wed May 31 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!uhog.mit.edu!rutgers!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!NewsWatcher!user From: wrengels@facstaff.wisc.edu (Bill Engels) Newsgroups: bionet.drosophila Subject: Re: PCR based P-element rescue Date: Wed, 31 May 1995 08:57:21 -0500 Organization: University of Wisconsin Lines: 18 Distribution: world Message-ID: References: <3qi1kc$nql@comet.EMBL-Heidelberg.DE> NNTP-Posting-Host: wrengels.genetics.wisc.edu In article <3qi1kc$nql@comet.EMBL-Heidelberg.DE>, Martin Zeidler wrote: > Does anyone have details of the primer seaquence and annealing temps for > the rescue of genomic DNA flanking a non-rescuable P-element insertion. 31 May 1995 I think the details you want are in Gloor et al., (1993, Genetics 135:81-95). \\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \ William R. Engels : WREngels@facstaff.wisc.edu Genetics Department, 445 Henry Mall : office: (608) 263-2213 University of Wisconsin : lab: (608) 262-5578 Madison, WI 53706 : FAX: (608) 262-2976 ---------------------------------------------------------------------- .