From owner-drosophila@net.bio.net Thu Jul 04 23:00:00 1996
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From: aghysen@ulb.ac.be (Ghysen Alain)
Newsgroups: bionet.drosophila
Subject: Re: Adult X-gal staining
Date: 5 Jul 1996 17:35:30 +0100
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Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
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Original-To: dros@dl.ac.uk

"Does anyone know how to do wholemount X-gal staining on
newly eclosed adult flies?"

Very young flies (or pupae once extracted from the puparium) ) can
be X-gal labelled by using the same protocol as for embryos, including
the heptane-fixative styep which presumably removes the waxy layer
that prevents penetration of the X-gal chemicals through the adult
cuticle. The protocol we use is described in Ghysen and O'Kane
Development 105, 35-52.


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