From owner-drosophila@net.bio.net Fri Aug 30 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!news.alt.net!news1.alt.net!news.aros.net!news.cs.utah.edu!news.cc.utah.edu!biol-21.biology.utah.edu!user From: rushton@biology.utah.edu (Emma Rushton) Newsgroups: bionet.drosophila Subject: wanted - 3rd Chromosome balancer with special properties Followup-To: bionet.drosophila Date: Fri, 30 Aug 1996 18:11:59 -0700 Organization: Biology Dept, University of Utah Lines: 22 Message-ID: NNTP-Posting-Host: biol-21.biology.utah.edu Dear Drosophilists, I am looking for 3rd chromosome balancers that will help us in our screen of mature embryos. We need to be able to distinguish our homozygous mutant embryos from homozygous balancer embryos at stage 16, without fixing or otherwise killing the embryos. One possibility would be to use a balancer which is reliably lethal at late larval or pupal stages, as we are interested in embryonic and early larval lethals. Another possibility would be to use a balancer which carries some very obvious mutation such as a segmentation mutation or strong head defect or something like snail, affecting early morphogenetic movements, which gives a strong late phenotype. If anyone has, or knows of such 3rd chromosome balancers, I will be eternally grateful if you tell me where to find them!!! Or if you have any other ideas, please let me know. Thanks a lot, Emma .