From owner-drosophila@net.bio.net Sun Oct 15 23:00:00 1995
Path: biosci!ucl.ac.uk!A.Gilchrist
From: A.Gilchrist@ucl.ac.uk
Newsgroups: bionet.drosophila
Subject: fixing tissues
Date: 16 Oct 1995 11:46:31 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9510161641.AA12346@onyx.gene.ucl.ac.uk>

I'm trying to prepare D melanogaster adult tissues (esp female reproductive
organs) for wax embedding and sectioning.

Does anyone have any ideas/opinions on which particular fixatives
(paraformaldehyde, glutaraldehyde, Carnoy's, methanol/acetic acid etc) work
well, bearing in mind that I DON'T want to do in situs, just histological
staining after the sectioning?  I'm more interested in preservation of
morphology than in treating the tissues for an in situ.  I can only seem to
find protocols relating to in situs and/or embryos.

Thanks in advance,

Stuart Gilchrist
ucbhasg@ucl.ac.uk


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