From owner-biotechniques@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 19(6) December 1995 Date: 10 Nov 1995 09:55:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 115 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01HXH8NKUEK29AQGA4@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 19(6) December 1995 Table of Contents BENCHMARKS Fast ion-exchange purification of a microsomal protein -Broverman, S.A. and Prestwich, G.D. Use of polylysine in solid phase protein sequencing -Beg, O.U. Extraction of RNA from Gram-positive bacteria -Magni, C., Marini, P. and deMendoza, D. Large-scale purification of plasmid DNA for biophysical and molecular biology studies -Baumann, C.G. and Bloomfield, V.A. Long-term preservation of DNA in agarose gels using 70% ethanol -Jacobs, D. and Neilan, B.A. Effects of ethanol concentration and incubation period at 65=F8C on CAT activity in mammalian cell extracts -Leahy, P., Carmichael, G.C. and Rossomando, E.F. Use of an uninterruptible power supply in a molecular biology laboratory -Rubin, S.A. Discriminating PCR artifacts using DHDA (directed heteroduplex analysis -Tang, J. and Unnasch, T.R. Qualitative low-level internal control for nested RT-PCR -Denis, M.G. and Lustenberger, P. Ligation- and PCR-based method for sequencing plasmid inserts -Goldsborough, A.S. and Beranger, F. PCR colony screening using the scintillation proximity assay to detect inserts in cloning vectors -Lerner, C.G. and Carter, C.D. Method to automatically produce bacterial cells at any phase of growth -Woeste, S., Baldwin, W. and Kirkish, M NEW FEATURE: The Internet On-Ramp (Cyberspace for Biologists) =20 SHORT TECHNICAL REPORTS Quantification of ribozyme-mediated RNA cleavage using silver-stained polyacrylamide gels -Palfner, K., Kneba, M., Hiddemann, W. and Bertram, J. Effects of lipopolysaccharide on transfection efficiency in eukaryotic cells -Weber, M., M=94ller, K., Welzeck, M. and Schorr, J. Modification of the TRI ReagentTM procedure for isolation of RNA =66rom polysaccharide- and proteoglycan-rich sources -Chomczynski, P. and Mackey, K. RESEARCH REPORTS Digital photography for the light microscope: Results with a gated, video-rate CCD camera and NIH-Image software -Shaw, S.L., Salmon, E.D. and Quatrano, R.S. Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of Escherichia coli K-12 -Feng, G. and Winkler, M.E. BIOCOMPUTING Using a world wide web server as a local organizer for protein and DNA sequences -Atwell, R., Gibbins, F. and Upton, C. Optimizing probe selection in directed heteroduplex analysis using HDProbe 1.1 -Zimmerman, P.A., Shapiro, M., Tang, J., Nutman, T.B. and = =20 Unnasch, T.R. Protein sequence interpretation using a spreadsheet program -Shaw, G. Efficient, automatic detection of heterozygous bases during large-scale DNA sequence screening -Phelps, R.S., Chadwick, R.B., Conrad, M.P., Kronick, M.N. and= =20 Kamb, A. PRODUCT APPLICATION FOCUS Oligonucleotide activation of type IIe restriction enzyme NaeI for digestion of refractory sites -Senesac, J.H. and Allen, J.R. 1995 AUTHOR INDEX (Volume 19) LITERATURE EXPRESS NEW PRODUCTS INDEX TO ADVERTISERS .