From owner-drosophila@net.bio.net Tue May 30 23:00:00 1995
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From: Martin Zeidler <zeidler@embl-heidelberg.DE>
Newsgroups: bionet.drosophila
Subject: PCR based P-element rescue
Date: 31 May 1995 15:22:52 GMT
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Does anyone have details of the primer seaquence and annealing temps for 
the rescue of genomic DNA flanking a non-rescuable P-element insertion. 
I assume such a protocol would involve the digestion and 
re-circularisation of genomic DNA with a selection of restriction 
enzymes followed by PCR amplification of the circularised DNA containing 
the P-element LTR sequences and its neighbouring genomic region. 
Any tips or information ?

Martin



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