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Subject: BioTechniques 20(1), January 1996
Date: 15 Dec 1995 10:08:37 -0800
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BioTechniques 20(1), January 1996
Table of Contents

BENCHMARKS

Device for the simultaneous screening of bacteriophage lambda
clones 
     -Espinosa, L. and Navarro, E.
Preparation of T-overhang vectors with high PCR product cloning
efficiency
     -Hadjeb, N. and Berkowitz, G.A.
Simple 5' extension method allowing transcript discrimination
     -Breda, C., El Turk, J. and Esnault, R.
Transfer technique for minimizing waste of sonified adjuvant
emulsions
     -Spack, E.G. and Toavs, D.
Removal of albumin from multiple human serum samples
     -Rengarajan, K., de Smet, M.D. and Wiggert, B.
Measurement of 8-OHdG in DNA by HPLC/ECD: The importance of DNA
purity 
     -Laws, G.M. and Adams, S.P.
Transient gene transfer into myotubes following differentiation
in culture
     -Robey, R.B., Osawa, H., Printz, R.L. and Granner, D.K.
Preparation of electro-competent E. coli using salt-free growth
medium
     -Sharma, R.C. and Schimke, R.T.
Rapid PCR method for site-directed mutagenesis on double-stranded
plasmid DNA
     -Xu, X., Kang, S.-H., Heidenreich, O., Li, Q. and           
      Nerenberg,M.
Measurement of human growth hormone using a chemiluminescence
assay and a "glow" luminometer
     -Guidon, P.T.,Jr., Peter, R. and Kumar, E.
Simple method for adapting DNA fragments and PCR products to all
of the commonly used restriction sites
     -Tsang, T.C., Harris, D.T., Akporiaye, E.T., Schluter, S.F.,
      Bowden, G.T. and Hersh, E.M.
Chemiluminescent detection of unique sequences on chromosomes
after on-slide PCR
     -Xie, H. and Troyer, D.L.
PCR-TIES (third irrelevant enzyme site)-mediated gene fusion
method
     -Zeng, M. and Lapeyre, J.-N.

NEW FEATURE: The Internet On-Ramp (Cyberspace for Biologists)

SHORT TECHNICAL REPORTS

Cloning strategies using a third irrelevant enzyme site (TIES) to
overcome certain cloning problems
     -Zeng, M., Huang, B.-R. and Lapeyre, J.-N.
Cloning of size-selected human immunoglobulin heavy chain rearrangements
from third complementarity-determining region fingerprint profiles
     -Raaphorst, F.M., Tami, J. and Sanz, I.E.
Gene transfer into subcultured endometrial cells using
lipofection
     -Lascombe, I., Mougin, P., Vuillermoz, C., Adessi, G.L. and
      Jouvenot, M.
Harvest protocol to reduce variability of soluble enzyme yield
from cultured cells
     -Buskin, J.N., Gregory, D.L., LaFramboise, W.A. and Hauschka, S.D.
Bicistronic vector for the creation of stable mammalian cell
lines that predisposes all antibiotic-resistant cells to express
recombinant protein
     -Rees, S., Coote, J., Stables, J., Goodson, S., Harris, S. and Lee, M.G.
Enhanced cytochemical detection of viral proteins and RNAs using
double-sided labeling and light microscopy.
     -Ding, X.S., Carter, S.A. and Nelson, R.S.

RESEARCH REPORTS

Expression of a bifunctional chimeric protein A-Vargula
hilgendorfii luciferase in mammalian cells
     -Maeda, Y., Ueda, H., Hara, T., Kazami, J., Kawano, G.,
      Suzuki, E., Nagamune, T.
Synthesis of a new substrate for detection of lacZ gene expression in live
Drosophila embryos
     -Minden, J.S.
Construction of a restriction map and gene map of the lettuce
chloroplast small single-copy region using Southern cross-hybridization
     -Mitchelson, K.R.

PRODUCT APPLICATION FOCUS

Vectors for expression and secretion of FLAG epitope-tagged
proteins in mammalian cells
     -Chubet, R.G. and Brizzard, B.L.

NEW PRODUCTS

INDEX TO ADVERTISERS

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